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KMID : 0368420020450020112
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Journal of Plant Biology
2002 Volume.45 No. 2 p.112 ~ p.117
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A Novel Tapetum-Preferential Gene from Nicotiana tabacum
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Seul, Jung Ah
Hong, Choo Bong/Lee, Il Ha
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Abstract
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The tapetum, a thin layer of cells in the anther, is important to the process of male gametogenesis. However, only a few of its functioning genes have been reported. Here we isolated a genomic clone, gNtA37, that showed tapetum-preferential expression. Transcript could be detected from the microspore mother-cell stage until the time that microspores were released. It coded a protein of 245 amino acids, with an intervening sequence of 176 nucleotides. This clone also carried a 5´ upstream region of 840 nucleotides and a 3´ downstream region of 430 nucleotides. Database searches for the open reading frame did not reveal any significant homology to previously reported nucleotide sequences or polypeptide sequences.
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